Translational Capabilities: Isolation of neural and glial cells (Milestone 4.E)
Timeline Start - End2016 - 2024
Research Implementation AreaTranslational Tools, Infrastructure, and Capabilities
Support the development of standardized, cost-effective, high throughput methods to isolate neural and glial cells for “omics” profiling and drug-screening.
Create a repository of fully characterized, quality controlled, and fully sequenced reference iPSC lines accessible to the wide research community that can serve as:
A standard control for healthy genotypes and for generating edited-isogenic lines carrying specific AD risk variants.
A source of cell lines from individuals from diverse cohorts with AD-related phenotypes and specific naturally occurring mutations/risk alleles.
A resource to test and provide reference data on protocols to generate all CNS cell types.
Summary of Key Accomplishments
NIA supports the development of Induced pluripotent stem cell (iPSC) resources as tools for basic and translational research through two targeted funding initiatives as well as investigator-initiated research. In addition, the research teams supported through the AMP AD program are developing iPSC resources and using them as essential tools for validation of novel targets. NIA-supported research led to the development of a collection of iPSC lines from 53 deceased individuals who had participated in two long-term clinical research studies: the Religious Order Study and the Memory and Aging Project. The cell collection comes from cognitively healthy individuals as well as those with AD. The cell lines have been characterized with molecular profiling and an array of biochemical assays measuring AD-related processes. These measurements were linked to the clinical/cognitive and pathologic data from the same individuals. This iPSC collection is a shareable resource of various types of brain cells that recapitulate person-specific AD-related biological processes.
The key accomplishments summary is current as of July 2022.
- RFA-AG-14-012: Human Cell Reprogramming for Functional Genetics of Alzheimer’s Disease (R01)
- RFA-AG-17-009: Impact of Aging in Human Cell Models of Alzheimer’s Disease (R01)
- PAR-18-516: Human Cell Biology of Alzheimer's Disease Genetic Variants (R01)
- RFA-AG-12-008: Human Cell Reprogramming for Aging and Alzheimer's Disease Research (R21)
- RFA-AG-21-005: Limited Competition: National Centralized Repository for Alzheimer's Disease and Related Dementias (NCRAD) (U24)
Research Programs and Resources
- Examples of Funded Projects
- National Centralized Repository for Alzheimer's Disease and Related Dementias (NCRAD)
- NIA Human Cell Reprogramming for Aging and Alzheimer’s Disease Research RFA Grantee Meeting
- UC Irvine ADRC iPSC Core
- UCSD ADRC iPSC Core