Available Tissues
Notice on F344 foundation stock.
Tissue availability at any point in time will be affected by the collection schedule and prior sales. Please contact the OBRRD Order Desk (rodents@nia.nih.gov) to inquire about tissue availability. Tissue is available from the following strains and ages:
| Strains | Ages available |
|---|---|
| F344 (Fisher 344) rats | 4, 12, 18, 24 and 28 months of age |
| F344-CDF | 4, 12 and 18 months of age |
| F344xBN F1 rats | 4, 18, 24 and 32 months of age |
| C57BL/6 mice | 4, 12, 18, 24 , 28 and 32 months of age |
| B6D2F1 mice | 4, 12, 18 and 24 months of age |
| BALB/cBy mice (females only) | 4, 12, 18 and 24 months of age |
| CB6F1 (BALB/c x C57BL/6)mice (males only) | 4, 18, 24 and 32 months of age |
| C57BL/6 mice caloric restricted and ad lib controls (males only) | 12, 18, 24 months of age |
| BALB/c mice, caloric restricted and ad lib controls | 8, 14, 20 and 24 months of age |
| F344 rats, caloric restricted and ad lib controls (males only) | 4, 18, 24, and 28 months of age |
| F344BN F1 hybrid rats, caloric restricted and ad lib controls (males only) | 4, 12, 18, 24, 34 months of age |
| B6D2F1 mice, caloric restricted and ad lib controls (males only) | 4, 12, 18, 24, and 28 months of age |
| Ames Dwarf mice and WT controls | 4, 12, 18, and 24 months of age |
| CBA mice | 4 and 24 months of age |
| DBA/2 mice | 4 and 21 months of age |
Animals are euthanized by CO2 asphyxiation and fresh, unfixed tissue and organs are flash frozen in LN2 and stored at –80 degrees Celsius. Special arrangements can be made for NIA-funded investigators for fixation of tissues or collection of additional ages or tissues such as bone. For information, contact Dr. Moro.
The following tissues will be collected from each animal:
- serum, 200 ul aliquots
- brain, whole or hemisphere
- cervical spinal cord
- eyes, pair
- sciatic nerves, pair
- heart, whole, rinsed in Phosphate Buffered Saline
- lungs, whole
- liver, whole
- spleen, whole
- ovary or testes, pair
- prostate (M), whole
- muscle – thigh,
- skin, shaved, from flank, approximately 1 x 1 cm square,
- kidneys, pair, whole
- adrenal gland, pair
- white adipose tissue - subcutaneous
- brown adipose tissue
- thymus, whole
- any tumors found
- pancreas
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