Research and Funding

Laboratory Analytes and Repository Plan - Health ABC

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  Examination  1
N=32121
Examination  2
N=27341
Examination  3
N=27341
Examination  4
N=2850 
Examination  5
N=2800 
Examination  6
N=2800 
Examination  7
N=2800 
Stored specimens:
1 extracted DNA, microtiter plates
cells for later transformation  
 
 
 
 
 
Stored specimens:
19 aliquots serum2
10 aliquots serum
4 serum
4 serum
 
10 serum
 
Stored specimens:
12 aliquots EDTA plasma3
10 aliquots EDTA
4 citrated
7 plasma
 
10 plasma
 
Stored specimens:
4 aliquots citrated plasma5
plasma
plasma*
 
 
 
 
Stored specimens:
3 aliquots urine4
4 aliquots citrated plasma  1 Vitamin C aliquot 4 urine aliquots
Cells for later transformation* platelets*
 
 
 
 
Stored specimens:
 
Platelets frozen from citrated plasma for mitochondrial DNA
 
 
 
 
 
Total specimens for storage in each examination:
139,087
94,238
12,101
12,000
 
11,400
 

1N includes 5% blind duplicates
2Aliquots = 13 aliquots 0.5 ml, 6 aliquots 1 ml.
3Aliquots = 8 aliquots 0.5 ml, 4 aliquots 1 ml.
4Aliquots = 2 ml, 1 acidified, 2 not.
5Aliquots = 0.5 ml
*plasma & platelets only collected from those who had cells collected in Year 3 (plasma sent to LCBR for pilot studies)

  Examination 1 Examination 2 Examination 3 Examination 4 Examination 5 Examination 6 Examination 7
Analytes Obtained:
Fasting glucose, insulin, two-hour (after glucola); glycated hemoglobin
Fasting glucose TSH, Free T-4 (in those with TSH alert)
Non-fasting, CBC Arterialized venous pH
Fasting glucose, glycated hemoglobin
 
Fasting glucose, glycated hemoglobin  Total cholesterol, HDL-cholesterol, triglycerides
 
Analytes Obtained:
Total cholesterol, HDL- and LDL- cholesterol, triglycerides, creatinine
 
 
Total cholesterol
 
 
 
Analytes Obtained:
Albumin, alkaline phosphatase
 
 
 
 
 
 
Analytes added:
Serum IL-6, TNF-a, CRP, leptin, PAI-1, Oxidized LDL
 
 
 
 
 
 
Analytes proposed:
Selected polymorphisms: ACE, IL-6, TNF-a, PPAR-8, RxR, HSP70-2, PAI-1 Selected musculoskeletal genes